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Transport and Metabolism of glycerophosphocholine by Candida albicans : Role of CaGIT3, CaGIT4, and CaGDE1
Author(s) -
Bishop Andrew C,
Ganguly Shantanu,
Cooley Ben,
Mitchell Aaron,
PattonVogt Jana
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.818.2
Subject(s) - candida albicans , permease , transporter , catabolism , metabolism , biochemistry , biology , flux (metallurgy) , microbiology and biotechnology , phospholipase d , regulator , chemistry , gene , enzyme , organic chemistry
Phospholipase B‐mediated turnover of phospholipids results in the production of glycerophosphodiesters. Candida albicans contains 4 ORFs ( CaGIT1–4 ) predicted to be glycerophosphodiester transporters. Here we have identified CaGit3 and CaGit4 as glyerophosphocholione (GroPCho) transporters, with CaGit3 having the greatest transport activity. Deletion of CaGIT2–4 abolishes the cells ability to utilize GroPCho as a sole phosphate source during early growth (<12 hours after inoculation) and abolishes short term (2 min) transport of [3H]GroPCho. Upon reintegration of CaGIT3 (orf19.1979) and CaGIT4 (orf19.1980), both growth and transport of GroPCho is rescued, albeit minimally for CaGIT4 . In addition, we have identified CaGDE1 (orf19.3936), as affecting the catabolism of internalized GroPCho, presumably by acting as a glycerophosphodiesterase. As is the case for CaGIT1 , the transcriptional regulator, CaPHO4 , regulates CaGIT3–4 expression. To our knowledge, this is the first evidence of a eukaryotic permease specific for GroPCho.