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Effect of gamma‐radiation and storage period on the erythrocyte membrane of the blood components
Author(s) -
Sousa Leilismara,
Maia Grazielle AS,
Silveira Alan B,
Cortes Vanessa F,
Santos Hérica L,
Barbosa Leandro A
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.817.1
Subject(s) - methemoglobin , chemistry , hemoglobin , erythrocyte membrane , irradiation , biochemistry , phosphatidylcholine , phosphatidylethanolamine , blood irradiation therapy , membrane , phospholipid , physics , nuclear physics
The use of γ‐radiation in blood products causes T lymphocytes inactivation, which mediated Graft‐versus‐host disease, preventing their development. Thus, it is interesting to understand the structural changes induced by irradiation and storage period, to provide the high quality of blood components. The CPDA‐1 bags of red blood cells (RBC) were separated into two units, being one irradiated with 25 Gy. An aliquot was removed for the experiments under sterile conditions on days 3, 5, 7, 9, 11, 14, 21 and 28. The major phospholipids detected in both samples were phosphatidylcholine and phosphatidylethanolamine, and the radiation caused modification of amount of all phospholipids compared to control samples. We also observed alterations in relation to the storage time and irradiation of cholesterol and methemoglobin levels, but oxyhemoglobin level and hemoglobin structure did not show difference. The Na,K‐ATPase activity decreased in 13% on day 7 and 40% on day 28. The thiobarbituric acid reactive substances level increased with the storage time‐dependent. The results indicated that γ‐radiation on RBC and their storage induced a possible reorganization of the lipid membrane content, but it does not appear to be the cause of structural changes in hemoglobin. Together, these alterations probably modulate the activity of important proteins and ionic homeostasis of irradiated RBC. Supported: FAPEMIG, CNPq, CAPES.