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Transport and trafficking of fluorescent sphingosine, sphingolipids, and their metabolites
Author(s) -
Kim Raehyun,
Kraft Mary L
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.814.6
Subject(s) - sphingolipid , sphingosine , bodipy , fluorescence , microbiology and biotechnology , fluorescence microscope , biochemistry , chemistry , intracellular , green fluorescent protein , biology , biophysics , receptor , physics , quantum mechanics , gene
Bioactive sphingolipids are located and transported in cells according to their metabolic pathway and cellular signaling events. However, absence of appropriate fluorescent tool has impeded understanding dynamic nature of sphingolipids during cellular function. To study sphingolipids trafficking and transport in live cells, it is the most desirable to use a fluorescent analog that follows native sphingolipid metabolism and transport, has high photostability, can be visualized in parallel with other common fluorescent labels, such as green fluorescent protein, and does not effect on cell viability. For this purpose, we recently reported a new fluorescent sphingosine, BODIPY 540 sphingosine, which satisfies these requirements. Here we use BODIPY 540 sphingosine to visualize the intracellular transport of fluorescent sphingosine and its fluorescent metabolites over time. We report the subcellular distribution of sphingolipids and their metabolites in living cells as a function of time. We also demonstrate that this probe allows visualizing the rapid movement of transport vesicles that contain fluorescent sphingolipids and their metabolites in real time.