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Label‐free Spectroscopic Imaging of Lipids in Live Cells and Intact Tissues
Author(s) -
Cheng JiXin
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.813.6
Subject(s) - raman spectroscopy , organelle , raman scattering , biophysics , lipid droplet , chemistry , microscopy , membrane , fluorescence , raman microspectroscopy , biochemistry , biology , optics , physics
Lipids are commonly visualized with the aid of fluorescent lipid analogs. These analogs do not provide compositional information about the organelles. Moreover, the labeling often perturbs the molecular properties due to the bulkiness of the tag. New platforms are being developed to overcome such barriers through using intrinsic molecular signals as contrast. In this presentation, label‐free imaging of lipids using signals arising from chemical bond vibrations will be demonstrated. By making use of the strong signal from C‐H stretch vibration, coherent Raman scattering microscopy allowed fast imaging of lipid droplet dynamics in live cells. Furthermore, by coupling fast coherent Raman imaging with spontaneous Raman spectral analysis of the fingerprint region, the composition of lipid droplets and the phase of lipid membranes are unveiled. These capabilities reveal previously underappreciated roles of lipids in health and diseases such as human cancers.