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Computational Model for Monitoring of the Membrane Potential with the Fluorescent Probe DiSC3(5)
Author(s) -
Lemeshko Victor V,
Alvarez Jose A.
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.802.1
Subject(s) - liposome , lipid bilayer , chemistry , vesicle , membrane , bilayer , fluorescence , membrane potential , biophysics , membrane fluidity , ionic strength , lipid bilayer phase behavior , aqueous solution , chemical physics , organic chemistry , biochemistry , biology , optics , physics
The optical properties of cationic dicarbocyanide dyes have been shown to depend on the transmembrane electrical potential of liposomes or living cells. In this work, we developed the most complete computational model allowing optimization of experimental conditions to monitor the membrane potential of membrane vesicles composed of neutral and charged phospholipids at fixed ionic strength of incubation media in a desirable voltage range. The model allows predicting potential‐dependent changes of the fluorescent properties of DiSC3(5) on concentration of the dye and on the lipid composition of the liposomes of certain sizes. It also takes into account the values of the surface potentials of the inner and outer leaflets of the lipid bilayer, as well as their changes due to the DiSC3(5) incorporation into the lipid phase. The probe dimerization in the aqueous and lipid phases, and the possibilities of the voltage‐dependent transitions of the dimers between two leaflets of the lipid bilayer and between the aqueous and lipid phases were also included in the model, in addition to the monomer transitions. The model was adjusted to several literature data obtained with DiSC3(5) probe as an indicator of the membrane potential in experiments with liposomes and biomembrane vesicles. Colciencias (Colombia) research grants #111840820380 and #111852128625.