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A neutron can make the difference: The 15 N stable isotope effect on metabolic pathways
Author(s) -
Filiou Michaela,
Webhofer Christian,
Nussbaumer Markus,
Landgraf Rainer,
Maccarrone Giuseppina,
Turck Christoph W
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.799.1
Subject(s) - metabolite , isotope , phenotype , metabolic pathway , metabolomics , enzyme , metabolism , chemistry , biochemistry , biology , gene , chromatography , physics , quantum mechanics
We have investigated the effects of metabolic labeling with the 15 N stable isotope in different organisms. Mice and bacteria were metabolically labeled using a 15 N‐labeled diet or 15 N‐labeled media, respectively. The 15 N‐labeled organisms were then compared with their unlabeled counterparts at the phenotypic and molecular levels with the help of behavioral tests, quantitative proteomics and targeted enzymatic, metabolic and immunochemical assays. At the phenotypic level, differences in behavioral traits between 15 N‐labeled and unlabeled mice were observed. At the molecular level, changes in protein expression, growth rates, pathway activities, metabolite levels and chromatographic peak patterns were found in the 15 N‐labeled specimens. Our results indicate for the first time a global 15 N isotope effect on labeled organisms. This information can be used to correlate phenotypic and molecular alterations and track relevant metabolic pathways. This work was supported by a QuantPro grant and the Max Planck Society