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Electrochemical Characterization of Superoxide Radical Chemistry and Scavenging by Natural Products
Author(s) -
Lee Grace,
Belli Stuart,
Caruso Francesco,
Rossi Miriam
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.794.12
Subject(s) - chemistry , superoxide , radical , hydroxyl radical , autoxidation , antioxidant , cyclic voltammetry , photochemistry , organic chemistry , electrochemistry , electrode , enzyme
The relationship between biological antioxidant molecules and superoxide free radical is a major curiosity. Superoxide radical is the leading contributor to ailments such as cardiovascular disease and cancer, and may play a role in the aging process. While it has been observed that antioxidants sequester these harmful radicals, the exact mechanism is still a mystery. In an effort to better understand the antioxidative properties that attribute to the sequestering of superoxide radical, the radical was studied in vitro in an electrochemical cell using cyclic voltammetry with a gold working electrode. Oxygen gas was converted into superoxide radical and stabilized in an aprotic solvent (DMSO) whereupon the resultant radical was exposed to various molecules thought to be beneficial to health. The effectiveness of said biomolecules was evaluated by quantifying the change in concentration of superoxide radical before and after exposure to antioxidants, as determined by calculating the change in the integral of the resultant currents produced within a set interval of voltage potential. Tocopherol (vitamin E), olive oil, grape seed oil, quercetin, 22–0 docosenoate, and nitro blue tetrazonium were tested for their antioxidant qualities. Spectrophotometry was used in conjunction with cyclic voltammetry to evaluate the effectiveness of certain antioxidants. Supported by HHMI 52006322 and VC URSI 2012