Premium
Spatial reorganization of yeast moonlighting protein, enolase, in hypoxia to alter carbon metabolism
Author(s) -
Miura Natsuko,
Shinohara Masahiro,
Tatsukami Yohei,
Morisaka Hironobu,
Kuroda Kouichi,
Ueda Mitsuyoshi
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.791.2
Subject(s) - enolase , glycolysis , cytosol , biochemistry , yeast , saccharomyces cerevisiae , hypoxia (environmental) , metabolism , enzyme , biology , alanine , microbiology and biotechnology , chemistry , oxygen , amino acid , immunohistochemistry , organic chemistry , immunology
Hypoxic conditions have critical effects on physiology of organisms. Using Saccharomyces cerevisiae , we have found that glycolytic enzymes conjugated with GFP formed cellular foci in hypoxic conditions. Enolase is one of foci‐forming glycolytic enzymes under hypoxia and is one of moonlighting proteins. Previously, we have found that N‐terminal part of enolase conjugated with EGFP formed cellular foci [1]. The foci formation of both full‐length and N‐terminal part of enolase was inhibited by single alanine substitution. In this study we tried to reveal the mechanisms of regulation and the biological function of enolase foci. The formation of enolase foci was inhibited by treatment with N‐acetylcysteine, AntimycinA and by knocking out SNF1 . Interestingly, after forming, foci were retained in the cell even in subsequent aerobic culture. Foci‐carrying cells in presence of oxygen could assimilate [U‐ 13 C]‐glucose more rapidly to produce aspartate, compared to cells which have no foci. These results suggest that in response to hypoxia, S. cerevisiae sensed mitochondrial ROS by activating SNF1/AMPK pathway to form complex of glycolytic proteins in cytosol [2]. The formation of foci may facilitate alternative use of carbon sources of hypoxia‐experienced cells. This work was supported by the Grant‐in‐ Aid for JSPS Fellows [09J02920 to NM].