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Thermal manipulation during chicken embryogenesis results in enhanced HSP70 gene expression and the acquisition of thermotolerance
Author(s) -
AlZghoul Mohammad Borhan
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.784.4
Subject(s) - hsp70 , hatching , andrology , embryogenesis , messenger rna , biology , gene expression , zoology , hormone , heat shock protein , endocrinology , medicine , chemistry , embryo , gene , biochemistry , microbiology and biotechnology
SUMMARY The aim of this study was to evaluate the effect of thermal manipulation (TM) at 38.5°C for 6 h or 18 h during embryonic days (ED) 10–18 on the muscle mRNA levels of heat shock protein 70 (HSP‐70) and the chick's ability to cope with the thermal challenge (TC; 41°C for 6h) at day 3, 7 and 42 of age. 750 fertile chicken eggs were obtained and divided randomly into 3 different treatment groups: control group was maintained at 37.8°C and 56% relative humidity (RH), TM 1 was subjected to TM at 38.8°C for 6 h and 65% RH during ED 10–18 and TM 2 was subjected to TM at 38.8°C for 18 h and 65% RH during ED 10–18. After hatching, the chicks from each treatment groups were randomly divided into 2 sub‐treatment groups (Naive and challenged). Chick's response to thermal challenged (41°C fro 6 hrs) was evaluated by determination of body temperature, plasma thyroid hormone levels (T 3 and T 4 ), plasma HSP70 and muscle mRNA levels of Hsp70. Our results indicated a long‐term improvement in the gene expression of HSP70 in the thermal manipulated chicks when compared with control, as manifested by a significant increase in the muscle mRNA levels of Hsp70 in thermally manipulated chicks during embryogenesis and during thermal challenge in post‐hatch chickens.