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Cadmium toxicity and small non‐coding RNAs in animal cells
Author(s) -
Estrella Luis A,
Cruz Andy W,
Negron Juan A
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.781.3
Subject(s) - cadmium , toxicity , viability assay , gene , biology , programmed cell death , gene expression , microbiology and biotechnology , cytotoxicity , cell , chemistry , genetics , in vitro , apoptosis , organic chemistry
Cadmium is an environmental hazard that causes cell damage. Cytotoxicity and cell death has been clearly demonstrated in liver and kidney. However, the specific toxic effect at the molecular level remains to be elucidated. In this study we have used Chinese Hamsters Ovary Cells (CHOs) to investigate the genetic and molecular mechanisms of cadmium toxicity. Cell viability assays, gene expression of metal binding proteins and non‐coding RNAs analysis have been done by Reverse Transcription Quantitative Polymerase Chain Reaction (RTqPCR). Exposition of CHOs to μM concentrations of cadmium, showed a correlation with cell death, overexpression of metallothioneins, and down‐regulation and up‐regulation and small non‐coding RNAs. These results suggest a role of small non‐coding RNAs in cadmium toxic effects in animal cells. This work was supported by US Department of Education, Award # PO31C110181.