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The role of NK1 receptor antagonist L‐703, 606 in the experimental traumatic brain injury
Author(s) -
Duan Hubin,
Hao Chunyan,
Li Shuzhen,
Fan Yimin
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.779.1
Subject(s) - calcitonin gene related peptide , substance p , pathological , medicine , antagonist , immunohistochemistry , receptor , endocrinology , h&e stain , traumatic brain injury , pathology , neuropeptide , psychiatry
Background and purpose L‐703, 606 is a NK1 receptor antagonist. the purpose of the research is to explore the the role of L‐703, 606 in the experimental traumatic brain injury£TBI£©. Methods 45 male Wistar rats were randomly divided into control group, L‐703, 606 intervention group (group L) ,TBI trauma group (group T). L‐703, 606 was injected into the tail vein of rats in group L after TBI. HE staining were used to observe the pathological changes in each group. The gene and protein expression of substance P(SP), calcitonin gene related peptide (CGRP) and nuclear transcription factor‐kappa B (NF‐¦ÊB) were detected by immunohistochemical staining and RT‐PCR. Results The gene and protein expression of SP, CGRP and NF‐¦ÊB in rat cerebral cortical lesions of group L and group T was increased compared with that of control group. Above indicators of group L was significantly reduced compared with that of group T. Only mild congestion, a small amount of inflammatory cell infiltration showed in group L. (P <0.05) Conclusion L‐703, 606 has a protective effect on the brain injury in rats. The possible mechanism maybe reducing the inflammatory reaction induced by SP and CGRP which released from cerebral cortical lesion.