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Influence of butyrate, a histone deacetylase inhibitor, on expression profile of microRNAs in vascular smooth muscle cells (VSMC)
Author(s) -
Ranganna Kasturi,
Mathew Omana P,
Milton Shirlette G
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.778.5
Subject(s) - butyrate , epigenetics , microrna , histone deacetylase , microbiology and biotechnology , histone deacetylase inhibitor , epigenetic regulation of neurogenesis , histone , cancer research , biology , carcinogenesis , cell growth , cancer epigenetics , chemistry , biochemistry , histone methyltransferase , gene , fermentation
Butyrate, a dietary‐derived epigenetic histone modifier and a histone deacetylase inhibitor, is an inhibitor of VSMC proliferation, a critical element in vascular remodeling and restenosis. Butyrate elicits cytoprotective, chemopreventive and chemotherapeutic activities through inhibition of cell proliferation, induction of cell death and/or cell differentiation by modulating gene expression via epigenetic changes. Because butyrate is an established epigenetic histone modifier, expression of certain microRNAs (miRNAs) may be altered by butyrate via epigenetic mechanisms including DNA methylation and histone modification. To explore this possibility, we examined expression profile of 650 miRNAs in butyrate inhibited rat VSMC proliferation by qRT‐PCR array platform. Our results indicate differential expression of 60 miRNAs. Among these, members of miRNA‐17–92 cluster are some of the miRNAs that are downregulated by butyrate in VSMC. Considering the role of miRNA‐17–92 cluster in oncogenesis and overexpression in different cancers, underexpression of members of miRNA‐17–92 cluster in butyrate arrested VSMC suggests antiproliferation action of butyrate is linked to corresponding increase in target genes of miRNA‐17–92 cluster, such as CDKN1A (p21Cip1). Overall, our miRNA expression data emphasizes role of miRNAs in antiproliferative and chemoprotective effects of butyrate in VSMC.