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MicroRNA in Leishmania RNA Virus 1
Author(s) -
Odera Joab Otieno,
PorterKelley Johanna
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.778.3
Subject(s) - mirbase , biology , microrna , rna , small rna , genetics , genome , leishmania , gene , rna virus , computational biology , virology , parasite hosting , world wide web , computer science
Leishmania RNA Virus 1 (LRV1) is a viral like particle found only in Leishmania species of the Amazon basin. microRNAs (miRNAs) are a large family of small endogenous RNAs, approximately 22 nucleotides long that regulate gene expression post‐transcriptionally in a complex process of binding to mRNA. Our hypothesis is that microRNAs are found in Leishmania RNA virus 1. The genome of Leishmania RNA Virus 1 Genome was retrieved from the National Center for Biotechnology Information Website. Sanger Institute‐miRBase Prediction software and subsequently, we chose five to further analyze based on E‐value and probable strand matches. The potential miRNAs data were consigned to the mFOLD software to determine their stem‐loop structures. Sanger Institute‐miRBase Prediction software revealed 500 potential miRNAs. Three of five selected miRNA sequences successfully showed miRNA stem loop structures that satisfied acceptable miRNA characteristics.