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Characterization Of CD‐209 Isoforms With Carbohydrate Recognition Domain Modified Due To Alternative Splicing
Author(s) -
Santos Luciana Lara,
Pereira Lailah Horácio Sales,
Taranto Alex Gutteres,
Junior Moacyr Comar,
Lopes Débora Oliveira,
Siqueira Jaqueline Maria
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.775.1
Subject(s) - gene isoform , alternative splicing , dengue virus , genbank , in silico , gene , homology modeling , biology , exon , homology (biology) , rna splicing , computational biology , biochemistry , microbiology and biotechnology , genetics , virus , enzyme , rna
The CD‐209 gene encodes a glycoprotein expressed as soluble or membrane, mainly present in human macrophages and dendritic cells. It is responsible for initial adhesion of the dengue vírus (DENV) to these cells. This gene undergoes alternative splicing, which results in thirteen different transcript of isoforms and consequently of the glycoprotein. The aim of this study is to predict the molecular structure of CD‐209 isoforms with carbohydrate recognition domain (CRD) modified due process of splicing alternative. The isoforms were obtained from the GenBank and aligned by Multialin software. Analysis in silico was performed to verify possible differences in CRD. 3D model structures were built by homology modeling (Swiss‐Model Server). We have demonstrated three sites of Ca2+ bind in CRD and amino acids residues that take part of it. We have found five isoforms with different CDR and only two of them keep three sites of Ca2+ bind. Validation steps were done and root‐mean‐square deviation values were found below 0.6. These variations may be correlated to the pathogenesis of dengue, since the virus needs of these sites of Ca2+ bind to interact with CD‐209 receptor. If the isoforms which miss some sites of Ca2+ are frequent in some populations this could confer a certain protection against virus infection. Future population studies would be interesting to better understand these issues. Support: FAPEMIG