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SWI/SNF remodels SIR3 heterochromatin during transcription
Author(s) -
Rege Mayuri M,
Peterson Craig L.
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.770.6
Subject(s) - euchromatin , heterochromatin , biology , swi/snf , transcription (linguistics) , genetics , chromatin , chromatin remodeling , saccharomyces cerevisiae , microbiology and biotechnology , gene , linguistics , philosophy
Heterochromatin in S. cerevisiae is assembled and maintained by the Sir proteins, primarily Sir2p, Sir3p, and Sir4p, at telomeres and the silent mating type loci. In addition to these canonically silenced loci, Sir3p is also found within euchromatin, where it appears to hinder gene transcription. Recent work from our lab has shown that the chromatin remodeling enzyme SWI/SNF, can evict Sir3p from nucleosomal arrays in vitro, and this activity can promote homologous recombination in vitro and in vivo. We have asked if SWI/SNF could also alleviate potential blocks to transcription caused by Sir3p. Consistent with this possibility, we find that the growth defects of a swi2Δ are partially suppressed by deletion of SIR3 . In contrast, inactivation of either SIR2 or SIR4 does not suppress the phenotypes of a swi2Δ. Gene expression analysis of swi2Δ and swi2Δ sir3Δ mutants have revealed a group of genes involved in cell‐cycle progression and cell wall organization in which SWI/SNF appears to control their expression by antagonizing Sir3p. Taken together, our studies suggest a new role for SWI/SNF to promote transcription via remodeling Sir3p in euchromatin.