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Three‐dimensional reconstructions of human embryos: vertebrobasilar system evolutive morphogenesis.
Author(s) -
LABROUSSE Marc,
LHUAIRE Martin,
MICARD Émilien,
SCHARBARG Emeric,
CENDRE Romain,
DELMAS Vincent,
FELBLINGER Jacques,
AVISSE Claude,
BRAUN Marc
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.755.2
Subject(s) - dicom , morphogenesis , anatomy , embryology , trichrome , computer science , biology , medicine , pathology , artificial intelligence , immunohistochemistry , h&e stain , biochemistry , gene
OBJECTIVES The purpose of this study was to describe the morphogenesis of the vertebrobasilar system from three‐dimensional reconstructions of 4 human embryos, and to compare our results with literature. MATERIALS AND METHODS We investigated four embryos belonging to the French collection of Paris‐Descartes University: 14mm (Carnegie stage 17), 15mm (CS 18), 22.5mm (CS 21) and 27mm (CS 23) respectively, serially sectioned. Staining was Masson's trichrome. Scanning was performed using an Epson V750 PRO ®, (resolution 2400 to 3200 dpi). Registration of image stacks and export in DICOM files were performed using original software developed by IADI Laboratory (INSERM U947, NANCY). The images obtained were exploited with ORS ® Visual. RESULTS From the embryo of 14mm to 27mm, the vertebrobasilar vascularization was well visualized. Its collaterals were seen. The main relationships with the mesencephalon and rhombencephalon were analysed. CONCLUSION This original software allows a new method of three‐dimensional reconstruction, and a better understanding of vertebrobasilar system evolutive morphogenesis. It brings a new perspective to the study of human embryology.