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The expression of thyroid hormone receptor mRNA and myosin heavy chain isoforms in mouse neonatal masseter muscle growth
Author(s) -
Miwa Yoko,
Sato Iwao,
Sunohara Masataka,
Shimada Kazuyuki
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.743.2
Subject(s) - myosin , masseter muscle , thyroid hormone receptor , myh6 , gene isoform , messenger rna , biology , hormone , myh7 , thyroid , medicine , endocrinology , mitochondrial biogenesis , receptor , gene expression , microbiology and biotechnology , gene , anatomy , myosin light chain kinase , mitochondrion , genetics
Objectives Thyroid hormone receptors (THRs) are composed of two isoforms, designated alpha and beta. It has been suggested that the thyroid hormone and THRs contribute to the regulation of masseter muscle development by affecting mitochondrial respiratory chain‐related biogenesis. However, the relationship between the mRNA transcript levels of THRs and myosin heavy chain isoforms (Myh1, Myh2, Myh4, Myh3, Myh6) in the mouse masseter muscle is unknown. Methods Quantitative real‐time RT‐PCR was used to measure the expression of THR mRNAs in the developing postnatal mouse masseter muscle. Results The expression of THR beta RNAs in the developing mouse masseter muscle decreased significantly from 0 to 10 days of age. Of the myosin heavy chains studied, Myh4 and Myh5 were the most highly expressed in the 10‐day‐old mice. Conclusions These data, along with the analysis of the RNA transcript levels of these genes, support a role for THRs as regulator of the growth and development of the postnatal mouse masseter muscle.