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DIFFERENTIAL GENE EXPRESSION PROFILES PRODUCED BY A HIGH FAT DIET IN WHITE AND BROWN ADIPOSE
Author(s) -
Faulkner Jessica L,
Gomolak Jessica R,
Garrett Michael R,
Didion Sean P
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.738.3
Subject(s) - adipose tissue , fold change , gene expression , medicine , endocrinology , gene , white adipose tissue , biology , gene expression profiling , brown adipose tissue , biological pathway , genetics
The goal of the present study was to determine gene expression profiles that change in response to a high fat diet (HFD) in white and brown adipose. Male C57Bl/6 mice were fed a control (10% kcal) or a HFD (60% kcal) for 12 wks. Genome‐wide expression profiles were examined in five adipose depots (epididymal (EPAT), perirenal (PRAT), subcutaneous (SCAT), perivascular (PVAT), subscapular (SSAT)) using the Affymetrix platform (GeneChip® Mouse 1.0 ST Array). Expression differences were analyzed using ±2‐fold change (P<0.05) and gene network analysis was performed using Ingenuity Pathway Analysis. In PVAT and SCAT, 21 and 45 genes respectively were found to be differentially expressed. In brown SSAT, 47 genes were differentially expressed; 25 of which were downregulated by 11‐ fold or greater and were classified as muscle‐specific. 130 and 41 genes were found to be differentially expressed in visceral EPAT and PRAT, respectively. In both EPAT and PRAT a number of genes related to inflammation were upregulated. Overall, GPR50 (an orphan receptor implicated in metabolism) had the greatest change in expression (29‐ & 60‐fold increase) in response to a HFD in PRAT and EPAT, respectively. In total, these data demonstrate that in response to a HFD there are gene expression profiles that are shared between adipose depots as well as genes/pathways that differentiate adipose types. Supported by NIH HL‐089884 & HL‐107632.