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STEM CELL FUNCTION IN ATHEROSCLEROTIC APOE −/− MICE
Author(s) -
Porto Marcella Leite,
Campagnaro Bianca Prandi,
Tonini Clarissa L,
Vasquez Elisardo C,
Meyrelles Silvana S
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.727.11
Subject(s) - propidium iodide , annexin , bone marrow , analysis of variance , apoptosis , flow cytometry , stem cell , phycoerythrin , andrology , medicine , immunology , endocrinology , microbiology and biotechnology , biology , biochemistry , programmed cell death
Background Stem cell (SC) has been used as a potential therapy for various diseases. However, the beneficial effects of SC are still a subject of controversy. Therefore, the aim of this study was to evaluate how aging and atherosclerosis affect bone marrow SC function in mice. Methods Female mice (n= 12) were divided into C57 and apoE−/− (2 and 18‐month‐old) groups. Bone marrow stem cells were isolated and obtained by immunomagnetic depletion. BM‐MNCLin‐ were stained with phycoerythrin (PE)‐conjugated antibody against Sca‐1 (stem cell antigen) or matched isotype control (anti‐rat IgG2a). For apoptosis detection, 105 BM‐MNCLin‐ were incubated Annexin V‐FITC and propidium iodide (PI). A FACSCanto II cytometer was used for the flow cytometric analysis. Data were acquired and analyzed using BD FACSDiva software. Data are mean±SEM. Statistical analysis was performed with two way ANOVA test. Results Flow cytometric analysis showed increased SC apoptosis in aged compared with young C57 animals (2m: 4,8 ± 0,5% vs. 18m: 39,9 ± 0,8%, p<0,05). This effect was pronounced in apoE−/− group (2m: 38,6 ± 0,3% vs. 18m: 44 ± 0,2%, p<0,05). Conclusion The present study demonstrated that aging and atherosclerosis impact on SC function, mainly when these two risk factors are associated. These results provide novel rationales for the optimal use of SC in therapies.