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Effect of surfactants on polystyrene nanoparticle (PNP) interactions with primary rat alveolar epithelial cell monolayers (RAECM)
Author(s) -
Kim Yong Ho,
Sipos Arnold,
Fazlollahi Farnoosh,
Borok Zea,
Kim KwangJin,
Crandall Edward D
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.722.5
Subject(s) - pulmonary surfactant , chemistry , poloxamer , biophysics , extracellular , zeta potential , intracellular , nanoparticle , biochemistry , materials science , nanotechnology , biology , organic chemistry , copolymer , polymer
We investigated effects of apical 0.1% (w/v) Pluronic (P) or 1 mg/mL Infasurf (I) on flux of 20 nm carboxyl‐modified (negatively charged) PNP (20‐PNP) or 20 nm amidine‐modified (positively charged) PNP (20+ PNP) across RAECM, across which the bioelectric properties (transepithelial resistance (Rt) and equivalent short circuit current (Ieq)) were unchanged in the presence of these two surfactants. Results of flux measurements, along with hydrodynamic diameter (HD) measured by dynamic light scattering and zeta potential (ZP) measured in the presence and absence of P or I, are summarized below.20+ PNP 20‐ PNPFlux (pg/cm 2 /sec) PNP alone 38±7 1.6±0.1 PNP with P 35±7 2.5±0.2 PNP with I 42±6 0.9±0.2 HD (nm) PNP alone 543±28 156±7 PNP with P 374±13 80±3 PNP with I 316±9 278±9 ZP (mV) PNP alone 37±10 −61±6 PNP with P 33±2 −29±2 PNP with I −44±1 −82±4These data indicate that there is remarkably little effect of P or I on PNP flux despite significant changes in measured HD and ZP. We conclude that PNP (and RAECM) exposure to either surfactant P or I surprisingly does not cause meaningful alterations in PNP translocation across RAECM, which may be explainable by the behavior of PNP in the extracellular microdomain adjacent to the apical alveolar epithelial cell membrane. (Funded by NIH and Hastings & Whittier Fdns.)