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Exercise does not increase skeletal muscle FNDC5 protein or mRNA in pigs
Author(s) -
Laughlin M. Harold,
Company Joseph M,
Booth Frank W,
Padilla Jaume,
Jenkins Nathan T,
Sacks Harold S,
Fain John N
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.713.2
Subject(s) - fndc5 , endocrinology , medicine , skeletal muscle , messenger rna , western blot , biology , chemistry , gene , cell , biochemistry , fibronectin
Endurance exercise training (EX) is reported to increase FNDC5 expression in skeletal muscle (SkM) of mice (Nature 481:463, 2012). We tested the hypothesis that EX increases FNDC5 expression in pig SkM. Castrated male Rapacz hypercholesterolemic (FH) and normal Yucatan miniature swine (Y) were divided into EX and unexercised (SED) groups. After 16–20 weeks pigs were anesthetized and tissue samples collected and FNDC5 gene expression measured by RT‐PCR. Citrate synthase activity (CS) (index of EX‐induced adaptation) was increased in SkM samples of both FH and Y EX. FNDC5 mRNA appeared 64% less deltoid and 25% less in long head of triceps brachii (LHT) SkM of FH EX pigs. EX did not appear to affect FNDC5 mRNA expression in fat or heart muscle. FNDC5 mRNA expression in subcutaneous fat was comparable to that in the SkM. EX enhanced PGC1α mRNA levels which were much higher in the SkM samples than in fat samples. The changes in PGC1α mRNA levels were not correlated with FNDC5 mRNA. Despite the increase in CS activity in SkM, there was no difference in FNDC5 protein content (Western blot) between SED and EX SkM of Y pigs. We conclude that EX does not increase FNDC5 mRNA or protein in pig SkM.