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Deterioration of mouse femur microstructure by heavy cigarette smoking and the effect of smoking cessation
Author(s) -
Chan Ruby Lok Yi,
Cho Chi Hin
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.713.10
Subject(s) - medicine , urine , smoking cessation , deoxypyridinoline , osteoporosis , femur , bone remodeling , passive smoking , bone mineral , smoke , physiology , alkaline phosphatase , dentistry , surgery , pathology , chemistry , osteocalcin , biochemistry , organic chemistry , enzyme
Smoking is a risk factor of osteoporosis. To investigate the dose effect of smoking, mice were subjected to ambient air, 2% or 4% cigarette smoke exposure for 14 weeks. To evaluate the effect of smoking cessation, ambient air or 4% cigarette smoke were given to mice for 20 weeks, while the smoking cessation group stopped smoking in the last 6 weeks. Urine and serum bone turnover markers; as well as femora and tibias mineral content, histology and microstructure were assessed. Deoxypyridinoline (DPD), minerals excretion in urine and serum alkaline phosphatase (ALP) activity increased after heavy smoking. Bone weight and mineral contents had no difference in all groups. Relative bone volume and trabecular thickness were lower with elevation in structure model index after 4% smoking. Smoking cessation returned body weight to control level. Serum ALP activity decreased after smoking cessation. Urine DPD level in smoking groups were significantly higher. Less intact trabecular structure in distal femur and reduced femur growth plate thickness were found in smoking groups. To conclude, chronic heavy smoking increases bone turnover and deteriorates bone microstructure in mice. Smoking cessation does not reverse the ill effects of chronic heavy smoking. The study was supported by research funds from the Jockey Club Centre for Care and Control and the Direct Grant, the Chinese University of Hong Kong.