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Sex‐based comparisons in oxidative DNA damage following 2h moderate cycling exercise
Author(s) -
Yasuda Nobuo,
Tanioka Toshihiro
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.712.9
Subject(s) - cycling , body mass index , creatinine , zoology , urine , medicine , chemistry , endocrinology , biology , archaeology , history
The purpose of the study was to determine the effects of 2h moderate cycling exercise on whole body oxidative DNA damage and repair in men and women. Recreationally active men (M, n=13; age: 19.9±1.4 year; height: 171.2±3.6 cm; body weight: 67.5±11.9 kg; body mass index: 23.0±3.5 kg/m 2 ; body fat: 15.1±6.6 %) and women (W, n=12; 20.±90.3 year; height: 160.1±5.6 cm; body weight: 54.7±5.8 kg; body mass index: 21.3±1.8 kg/m 2 ; body fat: 21.7±3.0 % [mean±SD]) served as the subjects. Before (Pre) and after (immediately Post and Post 24 hours) 2h cycling exercise at 60% VO 2 peak, spot urine samples were collected for later analysis of 8‐hydroxy‐2′‐deoxyguanosine (8‐OHdG) determined with high performance liquid chromatography. With regard to 8‐OHdG level (ng/mg Creatinine), two‐way (time × sex) analysis of variances (ANOVA) showed no significant main effects for time, sex or interaction (Pre: 3.0±1.2, Post: 3.6±1.2, Post‐24h: 3.2±1.1 for M; Pre: 3.2±1.2, Post: 3.5±1.1, Post‐24h: 3.2±0.9 for W). However, there was a tendency for 8‐OHdG to be higher following exercise and return to the baseline level at Post‐24h in both men and women. These findings indicate that men and women show similar whole body DNA damage and repair after 2h moderate cycling exercise, which appears not to induce cumulative harmful oxidative stress. Supported partly by funds from the Grant‐in‐Aid for Scientific Research (C) in Japan (Grant No.23500867).