Premium
Intramuscular doxorubicin accumulation and skeletal muscle fatigue: effects of prior exercise
Author(s) -
Quinn Colin,
Gibson Noah,
Bredahl Eric,
Schneider Carole,
Hayward Reid,
Hydock David
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.712.28
Subject(s) - skeletal muscle , medicine , doxorubicin , muscle fatigue , intramuscular injection , endocrinology , endurance training , saline , soleus muscle , chemotherapy , physical medicine and rehabilitation , electromyography
The anthracycline antibiotic doxorubicin (DOX) has been shown to accumulate in skeletal muscle and result in muscle fatigue, but the role that exercise plays in DOX accumulation and fatigue in skeletal muscle is unclear. The purpose of this study was to examine the effects of exercise prior to DOX administration on intramuscular DOX accumulation and skeletal muscle fatigue. Ten‐week old, male Sprague‐Dawley rats were randomly assigned to an endurance training group (treadmill training), a resistance training group (raised food/water), or a sedentary group for 10 weeks. After the activity treatment period, animals received DOX or saline as a control. One day after injection, the soleus and extensor digitorum longus (EDL) were excised, and fatigue was analyzed ex vivo . DOX levels in the soleus and EDL were then quantified using high performance liquid chromatography. The endurance and resistance training models employed attenuated the increased muscle fatigue associated with DOX treatment, but this reduction in fatigue was not associated with decreased intramuscular DOX levels. This suggests that the beneficial effects of exercise on DOX‐induced skeletal muscle fatigue observed one day following treatment are not necessarily attributed to reduced intramuscular DOX levels.