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Exercise regulates obesity‐induced chemerin expression in a tissue‐dependent manner
Author(s) -
Keslacy Stefan,
Gladstone Miriam,
Kim Yuho
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.712.12
Subject(s) - chemerin , adipose tissue , endocrinology , medicine , insulin resistance , gene expression , western blot , inflammation , obesity , biology , adipokine , gene , biochemistry
Chemerin has been involved in obesity‐induced inflammation and insulin resistance and is preconized as a new biomarker for diabetes. We previously showed that exercise modulates systemic chemerin, but the underlying mechanism is still unknown. Purpose To determine the source of the exercise‐induced changes in obesity‐related chemerin expression. Methods 44 mice (DIO C57Bl/6J) were separated into 2 cohorts: high fat diet (HFD) and normal diet (ND). Following a 12 week‐feeding period, diabetes‐relevant tissues (adipose, muscle, pancreas and liver) have been harvested. Chemerin gene expression and protein levels were assessed using pcr and western blot respectively. Results HFD resulted in enhanced chemerin gene expression in fat and muscle (P<0.005) as well as its receptor ChemR (P<0.05), but not in pancreas. Following exercise chemerin gene expression in fat did not change in ND but increased in HFD (P<0.05). We did not observe changes in chemerin or its receptor in muscle and pancreas following exercise. Our preliminary data suggested that exercise modulates chemerin protein expression in adipose tissue. Conclusion Our data suggest that the effect of exercise on obesity‐increased chemerin expression could be explained by adipose tissue. While chemerin gene expression increased in muscle with obesity, the effect of exercise seemed specific to adipose tissue. Study supported by Syracuse University.