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Comparative expression profiling of 50–60 year old male competitive athletes and lean healthy individuals
Author(s) -
Edgett Brittany A,
Mukherjee Kamalika,
St. Amand Tim,
Funk Colin D,
Gurd Brendon J
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.710.13
Subject(s) - medicine , lean body mass , insulin , endocrinology , aerobic exercise , gene expression , endurance training , athletes , biology , gene , physical therapy , body weight , genetics
The current study examined genetic and metabolic adaptations to aerobic exercise in highly‐trained masters athletes (MA; n = 9; age, 53 ± 3 yrs; BMI, 24 ± 3 kg/m 2 ; VO 2 peak, 59.1 ± 5.2 ml·kg −1 ·min −1 ) and age and BMI matched controls (CON; n = 8; 54 ± 5 yrs; 25 ± 3 kg/m 2 ; 35.9 ± 9.7 ml·kg −1 ·min −1 ). All participants performed a 45 minute endurance ride at 60% of their VO 2 peak followed by cycling at 90% VO 2 peak to fatigue. Blood was sampled before, immediately after, and 24 hours after exercise. Fasted insulin (MA, 18.1 ± 4.3; CON, 32.6 ± 13.8 pmol/L), HDL (MA, 1.91 ± 0.49; CON 1.28 ± 0.26 mmol/L), and lipid ratio (3.00 ± 0.62; CON 4.16 ± 0.86) were different between groups. MA also demonstrated an augmented insulin response to exercise compared to CON. A genome‐wide DNA microarray analysis of RNA from blood samples revealed that a variety of genes involved in insulin activity, cardiovascular and metabolic functions were significantly different between the two groups. These differences will be confirmed for individual changes in gene expression by quantitative real‐time PCR. These results may lead to new insights into signaling pathways that control the beneficial effects of exercise in older men, and may help to identify surrogate markers for monitoring exercise and training load. This research was supported by NSERC.