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Suppression of inflammatory cytokines in the kidney during chronic high salt intake in wild type and e‐NOS knockout mice
Author(s) -
Singh Purnima,
Castillo Alexander,
Majid Dewan S A
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.704.2
Subject(s) - endocrinology , medicine , tumor necrosis factor alpha , cytokine , proinflammatory cytokine , kidney , nitric oxide , knockout mouse , inflammation , nitric oxide synthase , chemistry , receptor
Inhibition of systemic nitric oxide (NO) production reduces interleukin (IL‐10) and increases tumor necrosis factor‐α (TNF‐α) level in the kidney. However, the status of inflammatory cytokines during chronic high salt (HS) intake, particularly in NO deficient condition, is not yet clearly known. In the present study, we measured the renal levels of pro‐ (TNF‐α and IL‐6) and anti‐ (IL‐10) inflammatory cytokines after HS (4% NaCl for 2 weeks) intake in both wild‐type (WT, C57BL/6) and e‐NOS knockout (KO) mice (~8 weeks old, n=6 in each group) and compared these findings with those in mice with normal salt intake (NS; 0.3% NaCl). The kidneys were collected under anesthesia at the end of experimental period and the cytokine levels were measured in homogenized renal tissues using ELISA kits. Mean arterial pressure (tail‐cuff plethysmography) was increased in KO (111±4 to 136±8 mmHg) but not in WT (99±5 to107±4 mmHg) after HS intake. The renal level of cytokines (expressed in pg/mg protein) during NS intake were significantly higher in KO (TNF‐α, 624±67 vs 325±73; IL‐6, 619±106 vs 166±61; IL‐10, 6087±567 vs 3929±378) compared to those in WT. However, HS intake significantly lowered the levels of these cytokines both in WT (TNF‐α, 114±17; IL‐6, 81±14; IL‐10, 865±130) and in KO (TNF‐α, 115±18; IL‐6, 56±7; IL‐10, 882±141). These data demonstrate that chronic HS intake suppresses both pro‐ and anti‐inflammatory cytokines levels in the kidney in intact as well as in eNOS deficient condition. (NHLBI #66432)