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Human mesangial cells treated with lipopolysaccharide mimicking human sepsis are able to produce catecholamines
Author(s) -
Magalhaes Dayane Cabo Grosso Peralta,
Arita Danielle Yuri,
Aragão Daniellle Sanches,
Almeida Waldemar Silva,
Casarini Dulce Elena
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.703.6
Subject(s) - lipopolysaccharide , tetrahydrobiopterin , extracellular , endocrinology , medicine , mesangial cell , chemistry , tyrosine hydroxylase , intracellular , sepsis , receptor , dopamine , kidney , biology , enzyme , cofactor , biochemistry
Mesangial cells (MC) have the entire machinery for the production of catecholamines (CA) and are able to express mCD14, a component of the receptor lipopolysaccharide (LPS) that is associated with type Toll like 4 receptor on the cell surface. The LPS in sepsis can trigger acute renal failure. We aimed to evaluate production/release of CA by immortalized human mesangial cell (HMC) treated with LPS. The HMC were cultured in DMEM with 10% fetal bovine serum. Then incubated with DMEM without FBS for the experiments in presence of LPS. The CA and cofactor tetrahydrobiopterin (BH4) were quantified using HPLC. The enzymes as tyrosine hydroxylase (TH), dopa decarboxylase (DDC) and dopamine ƒÒ‐hydroxylase (DƒÒH) were detected by Western blotting. Incubation of HMC with LPS 100 ƒÝg/ml increased intracellular levels of L‐DOPA and dopamine, and at the same time reduced the concentration of AD and L‐DOPA in the extracellular. There was an increase in expression of TH in HMC incubated with LPS 100 ƒÝg/ml that was correlated with the levels of CA. BH4 levels in extracellular compartment are directly related to increased levels of CA in the cells treated with LPS. CA are involved in various physiological processes in the kidney, being factors that impact on renal hemodynamics. Our results suggest that LPS can influenciate the production/release of CA in HMC. (supported by Fapesp and CAPES)