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Effect of diet on the progression of diabetic nephropathy in db/db mice
Author(s) -
Kandlikar Sachin S,
Joly Kristin M,
Horn Todd R,
Glascock Chris G,
Plato Craig F
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.702.13
Subject(s) - albuminuria , medicine , endocrinology , urine , creatinine , diabetic nephropathy , excretion , diabetes mellitus , nephropathy , renal function , urology
Diabetic nephropathy (DN) is the leading cause of end stage renal disease. Severity of diabetes and its complications are influenced by dietary intake while reduced renal mass (uninephrectomy, UNX) accelerates progression and worsens severity of DN. We hypothesized that high‐energy intake (HE) exacerbates the progression of DN in db/db mice. We studied long‐term interaction of HE and UNX on DN in db/db mice. Baseline water intake and urine volume were measured and assayed in male db/db and control mice (7–8 wks age). Depending on the diet [Purina 5008 (HE) or Harlan 8640 (normal chow, NC)] and surgical treatment (Sham or UNX), db/db mice were then functionally matched (10 wks age) into following five groups: 1) Control+NC, 2) Sham+NC, 3) UNX+NC, 4) Sham+HE, and 5) UNX+HE. Urine and blood samples, collected every 2 weeks, showed significant elevation in fasting blood glucose, HbA1c, and serum creatinine in all db/db compared to controls while urine creatinine was increased only in animals on HE. Water intake and urine excretion increased with age and were significantly higher in all db/db groups compared to controls. The fold change in 24‐hour urine albumin excretion compared to controls at 26 wks were 21.9 ± 8.6, 20.7 ± 5.4, 45.0 ± 7.5, and 60.2 ± 14.8 in groups 2, 3, 4, and 5 respectively. We conclude that HE combined with UNX exacerbates progression and severity of DN as indexed by albuminuria in db/db mice.