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Carboxylesterase 1 gene duplication and mRNA expression in adipose tissue are linked to obesity and metabolic function
Author(s) -
Friedrichsen Martin,
Poulsen Pernille,
Wojtaszewski Jørgen,
Hansen Peter Riis,
Vaag Allan,
Rasmussen Henrik Berg
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.701.6
Subject(s) - endocrinology , medicine , adipose tissue , insulin resistance , gene duplication , biology , gene expression , gene , obesity , genetics
Context and aims Carboxylesterase 1 (CES1) plays an important role in the control of the metabolism of triglycerides and cholesterol in various cell types including adipocytes and hepatocytes. In this study we investigated CES1 mRNA expression in adipose tissue as well as CES1 gene duplication and their association with measures of adiposity and metabolic function in a large twin population. Methodology 295 monozygotic and dizygotic twins (62–83 years) were subjected to a standard OGTT and excision of abdominal subcutaneous fat biopsies. Levels of CES1 mRNA and DNA copy number of the CES1 gene were assessed by quantitative PCR. Results CES1 mRNA expression in adipose tissue was positively associated with BMI ( P <0.001), insulin resistance ([HOMA], P =0.003) and fasting levels of glucose ( P =0.002), insulin ( P =0.006), and triglycerides ( P =0.003). Gene duplication of CES1 was not linked to altered CES1 mRNA expression ( P =0.63). Nonetheless, CES1 gene duplication was associated with increased glucose tolerance ( P =0.03) and decreased insulin resistance ( P =0.02). Conclusion CES1 mRNA expression in adipocytes as well as CES1 gene duplication were associated with measures of glucose metabolism, which raises the possibility of a potential role for this enzyme in the development of type 2 diabetes.