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Biotelemetry of intracranial pressure in freely moving rats: a detailed description of surgical implantation for chronic studies
Author(s) -
McLeod Damian,
Murtha Lucy,
Burke Julia,
Pitsillides Koullis
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.700.11
Subject(s) - intracranial pressure , medicine , skull , implant , pressure sensor , dura mater , anatomy , biomedical engineering , surgery , physics , thermodynamics
We aimed to develop a minimally invasive surgical technique for implantation of a biotelemetry device (EG3‐V0S1T, Transonic EndoGear Inc, Davis, CA), for chronic measurement of intracranial pressure (ICP) in freely moving rats. Under surgical anesthesia, a 2 cm midline scalp incision and blunt dissection exposes the skull surface. Two 1 mm shallow grooves are burred in the right and left parietal bones at 4 mm lateral to bregma for positioning of two 1 mm securing screws, which anchor a 2 mm layer of dental cement to the skull. Once dry, a 1 cm channel is burred through the dental cement, 2.5 mm lateral and parallel to the skull midline over one parietal bone. At the cranial end of the channel, a 2 mm hole is burred through the skull being careful to leave the meninges intact. The biotelemetry implant with battery connected is buried subcutaneously after making a 3 cm intrascapular skin incision. A 3F solid‐state pressure catheter, connected to the implant, is tunneled under the skin from the intrascapular region to exit at the scalp incision site. A 0.5 mm incision is made in the dura mater with a 30G needle tip and the pressure sensor head positioned under the dura. A thin layer of biocompatible caulking material is applied over the catheter head, and another layer of dental cement is applied to seal the cranial vault and ensure no movement of the pressure sensor head for chronic measurement of ICP.