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Acute hypoxia (AH) increases Fos‐IR in nNOS and AVP cells in the paraventricular nucleus of the hypothalamus (PVN)
Author(s) -
Coldren Kevin Max,
McCalmon Sean P.,
King T. Luise,
Kline David D.,
Hasser Eileen M.,
Heesch Cheryl M.
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.697.26
Subject(s) - medicine , endocrinology , vasopressin , forebrain , hypothalamus , microinjection , rostral ventrolateral medulla , chemistry , c fos , hypoxia (environmental) , nucleus , biology , central nervous system , medulla oblongata , neuroscience , gene expression , oxygen , biochemistry , organic chemistry , gene
Acute hypoxia activates peripheral chemoreceptors. Previously we reported that PVN‐projecting second order nTS neurons were activated (Fos‐IR) by AH. We hypothesized that, within the PVN, AH increases Fos‐IR in RVLM projecting, nNOS, and vasopressin (AVP) neurons. Microinjection of fluorogold (FG) in the RVLM retrogradely labeled RVLM‐projecting PVN neurons. Rats (n = 6) underwent either 3 h normoxia (N, 21% O2) or 3 h AH (10% O2). Immunohistochemistry was performed on forebrain sections and cell phenotypes were quantified. AH increased the total number of Fos‐IR cells (N= 24±2; AH= 118±14) at Bregma ~ −1.8 in the PVN. Also at this level, AH increased the number and % of nNOS cells that were activated (nNOS‐Fos) (N = 3±.0.4, 2± 0.4%; AH =15± 2, 12±1%). Activation of AVP cells (AVP‐Fos) tended to increase (P = 0.06) with AH (N = 1±.0.3, 2± 0.5%; AH = 5± 1, 7±2%). Although AH increased the number of nNOS cells more caudally (Bregma ~ −2.1), activation of RVLM‐projecting cells at either level of the PVN was unchanged by AH. These preliminary data suggest that activation of nNOS and AVP containing cells in the PVN contribute to cardiorespiratory responses to AH. NIH HL 98602.