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Sympathoexcitation and Pressor Response Induced by Central Amygdala‐injected Ethanol Requires Activation of Local NMDA Receptors
Author(s) -
Chen QingHui,
Gui Le,
Larson Robert A,
Chapp Andrew,
Keim Alexander,
Gu Mingjun
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.697.1
Subject(s) - nmda receptor , nbqx , chemistry , ethanol , glutamate receptor , pharmacology , receptor , endocrinology , medicine , biochemistry , ampa receptor
The Central Amygdala ( CeA) is involved in the sympathoexcitatory response to ethanol intake. However, the underlying neural mechanisms have not been determined. We tested the hypothesis that the increase in sympathetic nerve activity (SNA) in response to CeA‐injected ethanol requires activation of local NMDA receptors. In anesthetized rats, CeA ethanol (n=7) increased splanchnic SNA (SSNA, 121±21%), lumbar SNA (LSNA, 72±11%), and MAP (10±4mmHg) respectively. A cocktail containing KYN (7.2 nmol), a non‐selective glutamate receptor blocker, and ethanol attenuated responses (p<0.05; n=6) in SSNA (34±10%), LSNA (25±6%), and MAP (5±1mmHg) compared to that elicited by CeA ethanol alone (p<0.05). Similarly, responses to a cocktail containing AP5 (6 nmol), a NMDA receptor blocker, and ethanol in SNA and MAP were less than ethanol alone (p<0.05; n=6) and not statistically different from a cocktail containing KYN and ethanol. The symapthoexcitatory response to a cocktail containing NBQX (2.6 nmol), a non‐NMDA receptor blocker, and ethanol (n=6) were not different from CeA ethanol alone. CeA injection of KYN, AP5, and NBQX did not affect baseline parameters. Immunohistochemistry studies demonstrate abundant expression of NMDA NR1 receptors in CeA neurons with projections to the RVLM. This data indicates that sympathoexcitatory responses to CeA‐injected ethanol require action of local NMDA receptors. Support: AHA2640130 (QHC).