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Renal molecular reponses elicited by electrical stimulation of sympathetic renal nerve in wistar rats
Author(s) -
Pontes Roberto Braz,
Garcia Michelle L,
Veiga Glaucia RL,
OliveiraSales Elizabeth B,
Mirian A B,
Campos Ruy R,
Bergamaschi Cassia T
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.695.11
Subject(s) - losartan , reabsorption , epithelial sodium channel , endocrinology , renal sodium reabsorption , medicine , stimulation , aquaporin 2 , kidney , chemistry , angiotensin ii receptor type 1 , angiotensin ii , renin–angiotensin system , renal physiology , receptor , sodium , blood pressure , water channel , organic chemistry , engineering , inlet , mechanical engineering
This study evaluated the effects of acute sympathetic renal nerve electrical stimulation (RNES) over the expression of sodium (NHE3 and ENAC) and water (AQP2) transporters in the kidney in the presence or not of AT1 receptors angiotensin II blockade. Male Wistar rat (250g–300g), were divided in four groups: Sham (renal sympathetic nerve activity registered for 1h), RNES for 1h, [15V, 1Hz and 0,5 ms]), Sham+Losartan (30 mg/kg/day) and Losartan + RNES. Electrical stimulation produced increase in the NHE3 (1,6±0,2AU) gene expression and reduction in β‐ENaC (0,6±0,1AU) and AQP2 (0,6±0,1AU) expression. Animals just treated with Losartan did not presented differences at NHE3 expression but shows a reduction in ENAC (0,4±0,1AU) and AQP2 (0,3±0,1UA) expression compared with SHAM animals.. Treatment with Losartan+ RNES had no effect over the studied transporters. RNES produces alterations in sodium and water transporters in the renal tubule which can be involved in increased sodium reabsorption at the proximal tubule and reduced signalization to distal tubule reabsorption and these changes are angiotensin‐dependent. Supported by CAPES and Cnpq.