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Neuroprotective effects of EGCG on H2O2‐ and MPTP‐stressed PC12 cells
Author(s) -
Snitsarev Vladislav,
Andrej Anastasia,
Rotella David P
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.691.18
Subject(s) - mptp , neuroprotection , dopaminergic , chemistry , oxidative stress , parkinson's disease , pharmacology , dopamine , biochemistry , endocrinology , medicine , disease
Parkinson's disease results from degeneration of dopaminergic neurons. EGCG (epigallocatechin gallate), the most abundant catechin in tea and a powerful antioxidant, has been shown to play a neuroprotective role in the presence of degenerative stress inducers. We hypothesized that EGCG would protect a dopaminergic PC12 cell line from H 2 O 2 and MPTP (1‐methyl‐4‐ phenyl‐1,2,3,6‐tetrahydropyridine)‐induced stress. PC12 cells were plated at 10 4 cells/cm 2 in 60 (35 mm) wells. 15 wells were treated with 10 nM EGCG, 15 with 100 nM EGCG, and 15 with 1000 nM EGCG. 5 wells of each EGCG concentration were used as controls with no additional treatments. 5 wells of each EGCG concentration were treated with 100 μM H 2 O 2 , 5 wells of each EGCG concentration were treated with 10 μM MPTP, and 5 PC12‐ containing wells were used as the control with no treatment of EGCG. 5 wells were treated with 10μM MPTP only and 5 wells were treated with 100 μM H 2 O 2 only, for a total of 60 wells. The PC12 cells with respective treatments were incubated for 24 hours, and 4 random images of each well were taken, for a total of (60×4) 240 images. The above figures display PC12 cells treated with maximum concentrations of H 2 O 2 (100 μM) and EGCG (1000 nM) for 24 hours. The results indicate that EGCG plays a neuroprotective role in the presence of oxidative stress stimuli. Similar results were obtained after treating the PC12 cells with MPTP.