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Cerebral White Matter CD4+ T cell Infiltration is Associated with Hypertension in a rat model of HELLP Syndrome
Author(s) -
Wallace Kedra,
Morris Rachael,
Moseley Janae,
Evans Stephanie,
LaMarca Babbette
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.691.12
Subject(s) - hellp syndrome , preeclampsia , medicine , endocrinology , blood pressure , perfusion , mean arterial pressure , pregnancy , biology , heart rate , genetics
HELLP syndrome (hemolysis, elevated liver enzymes, low platelets) and preeclampsia (PE) (reduction in uterine perfusion pressure ‐RUPP), are hypertensive disorders that can occur during pregnancy with severe symptoms and consequences. The development of hypertension (HTN) may increase ischemia and inflammation in the maternal brain. We hypothesize that HTN in response to placental ischemia or HELLP increases CD4 + T cells expression in the maternal brain. On gestational day (GD) 12, sEndoglin (7ug/kg) and sFlt‐1 (4.7ug/kg) infusion began via miniosmotic pump into normal pregnant (NP) rats to induce HELLP. On GD14 RUPP rats had the lower abdominal aorta and ovarian arteries physically constricted. On GD18 carotid catheters were inserted (n=5/group), GD19 mean arterial pressure (MAP), plasma, tissue and maternal brains were collected. MAP increased from 96.2 ± 3.6 in NP rats to 127.8 ± 3.8 (p=.05) in RUPP rats; to 113.4±3.2mmHg in HELLP rats (p=.05). RUPP and HELLP rats had increased circulating CD4 + T cells compared to NP rats (p=.01, p=.05). CD4 + T cells along the corpus callosum in RUPP (136.1 OD) and HELLP rats (151.9 OD) were increased compared to NP (114.5 OD, p=.05, p=.05). These data indicate that CD4 + T cells are increased in both the circulation and the brain and may therefore be one potential link in the neuropathophysiology of HTN associated with PE and HELLP syndrome.