Premium
Oxytocin affects nitric oxide and cytokine production by sepsis‐sensitized macrophages
Author(s) -
OliveiraPelegrin Gabriela Ravanelli,
Saia Rafael Simone,
Cárnio Evelin Capellari,
Rocha Maria José Alves
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.690.3
Subject(s) - sepsis , nitric oxide , endocrinology , medicine , oxytocin , nitrite , secretion , proinflammatory cytokine , tumor necrosis factor alpha , immune system , cytokine , lipopolysaccharide , macrophage , inflammation , chemistry , immunology , biochemistry , organic chemistry , nitrate , in vitro
Oxytocin (OXT) secretion during cecal ligation puncture (CLP)‐ induced sepsis has not yet been examined. Although immune properties have been attributed to OXT, its effect on CLP‐sensitized macrophages has never been investigated. We analyzed the OXT secretion during CLP and its effect in CLP‐sensitized macrophage cultures. Male Wistar rats were decapitated 4, 6 or 24h after CLP surgery or sham operation and blood, brain and neurohypophyses collected for OXT measurements. In another set of animals we studied the effect of OXT on nitrite, TNF‐α, IL‐1β and IL‐10 production of peritoneal macrophages harvested at 6 and 24h after CLP. In the early phase of sepsis (4–6h), OXT levels increased in plasma and decreased in hypothalamus and neurohypophysis. In the late phase (24h), plasma and neurohypophyseal levels remained basal. In the PVN, the OXT content remained low, but in SON increased. Macrophages of the early phase of sepsis pre‐treated with OXT and LPS‐stimulated showed decreased nitrite, TNF‐α and IL‐1β levels, but no alteration in IL‐10 production. In the late phase they showed reduction only on IL‐1 β. OXT secretion during sepsis may represent a neuroendocrine response contributing to the overall host response to infection by decreasing the proinflammatory response and oxidative stress. Financial support: FAPESP, CNPq/PROCAD