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Anti‐aggregatory effects of Sulforaphane on in vitro platelet function
Author(s) -
Gillespie Scarlett,
O'Malley Aislinn,
Solomon Antonia,
Emerson Michael,
Gavins Felicity
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.686.6
Subject(s) - platelet , chemistry , sulforaphane , lactate dehydrogenase , incubation , platelet rich plasma , pharmacology , in vitro , cytotoxicity , biochemistry , immunology , medicine , enzyme
Sulforaphane (SFN) is a phytochemical with proven anti‐inflammatory effects in various inflammatory models. Platelet‐leukocyte‐endothelial cell interactions are integral to the inflammatory cascade. Research has investigated SFN's effects on leukocytes and endothelial cells, but its effects on platelets are previously unknown. Aim Elucidate the effect of SFN on platelets in‐vitroMethods and Results Platelet aggregation was studied using light transmission aggregometry. Washed platelets (WP) were incubated with SFN (1, 40, 60 and 100μM) for 2/30mins, then challenged with collagen (2.5μg/ml). Incubation with SFN, 2 min (100μM) and 30min (40, 60 and 100μM), prior to collagen‐stimulated decreased platelet aggregation. Similar trends were seen following SFN incubations with platelet rich plasma (PRP) aggregations to ADP (1.56μM), however, this was not significant. Ca 2+ mediated platelet signalling showed no detectable Ca 2+ mobilisation following SFN addition. Lactate dehydrogenase (LDH) release following SFN incubation showed negligible differences to that associated with vehicle treatment or agonist stimulation. Conclusion SFN inhibited platelet aggregation to collagen in WP, and showed similar trends in PRP following ADP stimulated aggregations. SFN incubation did not appear to be associated with calcium mobilisation events, indicating these anti‐aggregatory effects were independent of intracellular Ca 2+ movement, and low level LDH release suggested these effects were not via cytotoxicity. This novel work indicates an anti‐aggregatory ability of SFN in an agonist specific manner. Work funded by the Medical Research Centre, UK.