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Tumor Cell Interaction with Sprouting Blood Vessels in 3D Microfluidic Platform
Author(s) -
Ahn Song Ih,
Song Sukhyun,
Shin Jennifer Hyunjong
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.685.22
Subject(s) - intravasation , metastasis , blood vessel , circulating tumor cell , microbiology and biotechnology , biology , cell , vascular endothelial growth factor , endothelial stem cell , pathology , cancer cell , cytoskeleton , cancer , chemistry , cancer research , medicine , vegf receptors , endocrinology , biochemistry , genetics , in vitro
Tumor cells interact with blood vessels in the tissue in a number of different ways. For example, intravasation, tumor cell invasion into the blood vessel, is a critical step in early stage of cancer metastasis. Although previous studies have reported the tumor cell‐endothelial cell interactions in endothelial barrier, the interactions in the hyper‐sprouting tumor vasculature are not fully characterized. In this study, a novel microfluidic platform was developed to observe TC‐EC interaction between invading metastatic tumor cells and sprouting blood vessel in real‐time. Directional migration of metastatic breast tumor cells is induced by epidermal growth factor (EGF) gradient. Sprouting lumenized blood vessels are generated by the biochemical gradient of vascular endothelial growth factor (VEGF) and the secreted proteins from fibroblasts. Disruption of endothelial junction and cytoskeletal changes were observed during the tumor cell invasion into blood vessel. We also test the role of a few therapeutic biochemical reagents on the regulation of TC‐EC interactions.