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Renin/prorrenin receptor expression on leukocytes associated with an experimental model of preeclampsia
Author(s) -
SanchezGuerrero Erika,
HernandezCampos Maria Elena,
TrinidadGomez Margarita Itzel,
LopezSanchez Pedro
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.684.4
Subject(s) - preeclampsia , renin–angiotensin system , medicine , endocrinology , receptor , flow cytometry , spleen , immune system , basal (medicine) , pregnancy , chemistry , biology , immunology , blood pressure , genetics , insulin
Renin/prorrenin receptor (RPR) is an important component of the renin‐angiotensin system; this system plays a relevant role in blood pressure regulation and electrolyte balance. RPR is a single‐spanning membrane protein that binds both renin and its inactive precursor, prorrenin. Binding to RPR increases the catalytic activity of renin and induces non‐proteolytic activation of prorrenin, leading to angiotensin‐dependent responses. The presence of RPR on immune cells and if this presence plays a role during preeclampsia is unknown. The aim of this work was to determine the expression of RPR on leukocytes from spleen during normal pregnancy and pregnancy complicated with preeclampsia in rats. We used female Wistar rats groups: 1) non‐pregnant rats, 2) pregnant rats, 3) non‐prengnant rats whit subrenal aortic coarctation, 4) pregnant rats with subrenal aortic coarctation (preeclamptic rats). We used flow cytometry, inmunoblot and RT‐PCR to determine RPR expression upon leukocytes from spleen. We found similar, basal expression of RPR in groups 1–3; however, preeclamptic rats expressed this receptor in a lower amount. These suggest a possible role of RPR in PIH. Supported by SIP‐IPN 20120122 and 20120929 grants