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Renal ischemia‐reperfusion (I/R) injury induces a rapid activation of local inflammatory markers and causes increased peritubular permeability.
Author(s) -
Tenstad Olav,
Bivol Liliana Monica,
Iversen The late Bjarne Magnus,
Hultström Michael,
Reed Rolf Kåre,
Wiig Helge
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.682.10
Subject(s) - peritubular capillaries , lymph , kidney , ischemia , endocrinology , chemistry , cytokine , medicine , renal ischemia , renal cortex , reperfusion injury , renal blood flow , pathology
Local cytokines were measured in renal hilar lymph and in cortex after 30 min unilateral occlusion of renal artery followed by 120 min reperfusion. The effect of I/R on size selectivity for proteins in glomerular and peritubular capillaries were estimated using HPLC and mass spectrometry. Whereas all measured mediators increased dramatically in renal hilar lymph, plasma and renal cortical tissue samples and approached control levels after 120 min reperfusion, the responses were differentiated. IL‐1beta, MCP‐1 and leptin were markedly increased in plasma before reperfusion, reflecting an extra renal response possibly induced by afferent renal nerve activity from the ischemic kidney. The cortical cytokine content also reached high levels before reperfusion suggesting proteolytic release of local stores. However, only TNF‐alpha showed increased lymph to plasma ratio (L/P>;1) following 30 min reperfusion indicating release of mediators directly into the bloodstream in agreement with abundant cytokine staining of glomerular and peritubular capillaries. The cytokine levels were paralleled by a significant increase in high molecular weight plasma proteins in both lymph and urine indicating temporarily reduced size selectivity of both glomerular and peritubular capillaries. In conclusion, short time renal ischemia is a strong trigger for a local as well as systemic inflammatory reaction.