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The mechanism of protective effects of branched‐chain amino acids on hepatic ischemia/reperfusion‐induced liver injury in rats
Author(s) -
Kitagawa Tomomi,
Yokoyama Yukihiro,
Kokuryo Toshio,
Nagino Masato
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.682.1
Subject(s) - liver injury , microcirculation , lipopolysaccharide , cell adhesion molecule , kupffer cell , endothelin 1 , reperfusion injury , medicine , liver function , pharmacology , endocrinology , ischemia , chemistry , immunology , receptor
We determined whether there is a protective effect of branched‐chain amino acid (BCAA) on hepatic ischemia/reperfusion (IR)‐induced acute liver injury. Wister rats were divided into four groups: simple laparotomy with vehicle; simple laparotomy with BCAA; IR (30 min clamp) with vehicle; and IR with BCAA. Serum liver function tests and the gene expression of adhesion molecules and endothelin‐1 in the liver were examined. In the in vivo study, portal venous pressure, leukocyte adhesion, and hepatic microcirculation were evaluated. Furthermore, Kupffer cells were isolated and cultured with BCAA in lipopolysaccharide (LPS). Increased levels of liver function tests, the expression of adhesion molecules and endothelin‐1, portal venous pressure, enhanced leukocyte adhesion and deteriorated hepatic microcirculation following IR were significantly attenuated by BCAA treatment. In the experiment using isolated Kupffer cells, the expression of inflammatory cytokine, and endothelin‐1 in response to LPS stimulation was attenuated by BCAA. Oral administration of BCAA has excellent therapeutic potential for IR‐induced liver injury. These effects may result from the direct attenuation of Kupffer cell activation.