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Relating Lymphatic Vessel Mechanical Properties Measured in Vitro to Regulation of Interstitial Fluid Volume in Vivo
Author(s) -
Pariseau Hugo,
Franks Jonathan D.,
Lipinski Andrew E.,
Dongaonkar Ranjeet M.,
Quick Christopher M.
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.681.8
Subject(s) - lymphatic system , interstitial fluid , preload , afterload , lymph , in vivo , chemistry , starling , anatomy , biomedical engineering , lymphatic vessel , lymphedema , hydrostatic pressure , mechanics , materials science , cardiology , pathology , medicine , hemodynamics , biology , physics , microbiology and biotechnology , cancer , breast cancer , metastasis
Although lymphatic vessels are structured similarly to veins, lymphangions, the segments of lymphatic vessels between valves, behave like ventricles. Their spontaneous contraction propels lymph from the low‐pressure interstitium to higher‐pressure veins. Lymphangion pumping in vivo affects transmural pressure and flow, and the afterload of each lymphangion forms the preload of the next. Because lymphangions are sensitive to mechanical stimuli, the basic mechanisms influencing function are studied in vitro, where inlet and outlet pressures can be rigorously controlled. However, the relevance of in vitro results for clinical research has yet to be established. We therefore developed a mathematical model to determine the impact of the interaction of a lymphangion with its environment on interstitial fluid volume. We first characterized microvascular filtration into the interstitium with the Starling‐Landis equation. Lymph flow from the interstitium into the lymphatic network leading to the lymphangion of interest was characterized by modifying the Drake‐Laine equation. The lymphangion itself can be modeled empirically from in vitro data. Lymphangion outlet pressure was assumed to equal central venous pressure. With the ability to simulate diverse edemagenic conditions, our novel approach translates basic lymphatic research into clinically relevant edema research.