Premium
VEGF‐C induces lymphangiogenesis in the rat mesentery culture model
Author(s) -
Sweat Richard Shelton,
Lee Murfee Walter
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.681.10
Subject(s) - lymphangiogenesis , mesentery , lymphatic system , lymphatic endothelium , angiogenesis , vegf receptors , vascular endothelial growth factor c , pathology , biology , chemistry , anatomy , medicine , microbiology and biotechnology , cancer research , vascular endothelial growth factor a , vascular endothelial growth factor , metastasis , cancer
Recently, the rat mesentery culture model was established as a tool for investigating multi‐cellular dynamics involved in angiogenesis. Cultured tissues displayed intact blood and lymphatic microvascular networks. The objective of this study was to determine whether VEGF‐C induces lymphangiogenesis in this model. Mesenteric tissue windows were harvested from adult male Wistar rats and cultured for 5 days in either serum‐free minimum essential media (MEM) or MEM supplemented with VEGF‐C (100 ng/ml). Tissues were then labeled for PECAM and LYVE‐1. VEGF‐C treatment caused a significant increase in the number of lymphatic sprouts per lymphatic network area compared to MEM controls (p < 0.01). Lymphatic sprouts were observed along the hierarchy of intact lymphatic networks and were PECAM and LYVE‐1 positive. These results suggest the rat mesentery culture model can be used to investigate the interrelationships between lymphangiogenesis and angiogenesis in a controlled environment.