Premium
Effect of surface charge and magnetic field on iron oxide nanoparticle permeability in a cell culture model of the blood brain barrier (BBB)
Author(s) -
Sun Zhizhi,
Yathindranath Vinith,
Worden Matthew,
Hegmann Torsten,
Miller Donald
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.668.1
Subject(s) - permeability (electromagnetism) , surface charge , iron oxide nanoparticles , nanoparticle , magnetic nanoparticles , paracellular transport , tight junction , chemistry , biophysics , materials science , membrane , nanotechnology , biochemistry , biology
Surface charge and applied magnetic fields affect cellular uptake of iron oxide nanoparticles (IONPs). However, their effects on permeability of IONPs across the BBB remain unexplored. The aim of this study was to determine the impact of IONP surface charge on permeability in a cell culture model of the BBB in the presence and absence of a magnetic field. Permeability of positively (AmS‐) and negatively charged (TMSPEDT)‐IONPs was evaluated in confluent bEnd.3 monolayers grown on polycarbonate membrane inserts (3 μm pore). Our results show neither IONPs were not transported across the monolayer even in presence of magnetic field. When tight junctions were disrupted using D‐mannitol, 44% and 28% of TMSPEDT‐IONPs were found across the monolayer in presence and absence of magnetic field, respectively. Under the same conditions, there was only 10% permeability of the AmS‐IONPs detected. In conclusion, negatively charged IONPs have a more favorable permeability profile in brain endothelial cells via paracellular route following osmotic disruption. Use of TMSPEDT‐IONPs during transient disruption of BBB, may improve nanoparticle based drug delivery to the brain. Support provided by Manitoba Medical Services Foundation, Thorlakson Foundation and NSERC.