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The ERK/MAPK‐Dependent Pressor Effect Of Intra‐RVLM Ethanol Is Tonically Attenuated By Local Phosphatases In Normotensive Rats
Author(s) -
ElMas Mahmoud M,
AbdelRahman Abdel A
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.654.7
Subject(s) - rostral ventrolateral medulla , medicine , phosphatase , chemistry , endocrinology , microinjection , mapk/erk pathway , ethanol , kinase , medulla oblongata , phosphorylation , central nervous system , biochemistry
We recently reported that microinjection of ethanol into the rostral ventrolateral medulla (RVLM) elicits a modest pressor response in conscious normotensive rats. In this study, we tested the hypothesis that the RVLM phosphatase/extracellular signal‐regulated kinase (ERK) pathway modulates the pressor effect of ethanol. We show that the modest pressor response elicited by intra‐RVLM ethanol (10 μg) was: (i) abolished following local ERK inhibition with PD98059 (1 μg); (ii) associated with significant reduction in local phosphatase activity. Inhibition of the RVLM ser/thr phosphatase activity by okadaic acid (OKA, 0.4 μg) caused significant increases in blood pressure (BP) and potentiated the magnitude and duration of the pressor response as well as the phosphatase inhibition elicited by subsequent intra‐RVLM administration of ethanol. Intra‐RVLM acetaldehyde (2 μg), the main metabolic product of ethanol, caused no changes in BP or RVLM phosphatase activity but it produced significant increases in BP and inhibition of local phosphatase activity in OKA‐pretreated rats. Together, the RVLM phosphatase activity acts tonically to attenuate the ERK‐dependent pressor effect of ethanol or acetaldehyde in normotensive rats.