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Characterization of endometrial scarless healing using a postpartum mouse model
Author(s) -
Yoshii Asuka,
Kitahara Shuji,
Ezaki Taichi
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.650.5
Subject(s) - endometrium , angiogenesis , wound healing , uterus , medicine , hypoxia (environmental) , rat model , andrology , surgery , chemistry , oxygen , organic chemistry
The repair of endometrium is interesting for the remarkable speed without any scars, the process of which differs from ones in general wound healing. We sought to characterize its healing mechanism using an experimental mouse model. In this model, ICR mice had a Caesarean operation on Day 18 (D18) of gestation. On D1 after operation, a massive leakage of intravenously injected lectin towards the luminal side of the uterus was seen, indicating a continuous bleeding from cut ends of injured vessels. The leakage stopped by D2 may due to the involution by myometrial constraction. The endometrial epithelium completely recovered by D5 without any cicatrization. Surprisingly a large amount of fibrin in the injured endometrium disappeared as arginase1‐positive macrophages increased in and around it. Furthermore, the immunostainings of HIF1a as a marker of factor induced by hypoxia and VEGF revealed that angiogenesis occured in the endometrium before the completion of endometrial repair. We suggest that the change of oxygen concentration due to the rapid myometrial contraction might be involved in triggering the endometrial scarless healing.