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Proteomic analysis of microparticles produced by wound healing myofibroblasts cells
Author(s) -
Langlois Amélie,
Larochelle Sébastien,
Siagh Samir,
Genest Hervé,
Moulin Véronique J
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.649.13
Subject(s) - proteome , depolymerization , chemistry , biochemistry , wound healing , cytoskeleton , microbiology and biotechnology , biology , genetics , organic chemistry , cell
Production of microparticles (MP) by myofibroblasts (Wmyo) during wound healing process is a new finding in the field of cellular communication. To determine the proteome of MP, the 2D‐DIGE method was performed over MP produced by 6 different populations of Wmyo. Each gel allowed us to analysed MP extravesicular proteins and total protein content, due to the use of different Cydye labelling methods. Fluorescent labelling of each gel were acquired using Typhoon Variable Mode Imager system and all images were analysed by Delta2D software. We were looking for highly expressed and extremely conserved proteins. With these parameters and Saffold Viewer software, 133 spots were sent to mass spectrometry analysis for further characterisation. 292 different proteins were identified with a 95% probability of being peptides unique to one protein. This wide variety of proteins included several enzymes such as fructose‐biphosphate aldolase A, L‐lactate dehydrogenase and ATP synthase. Lactadherin, Annexin A2, filamin‐A serpin H1 and elongation factors were also revealed in all samples studied. A large proportion of all the protein listed corresponded to cytoskeleton‐related protein. Knowledge of the protein content of MP used by Wmyo to communicate between each other will enable a better comprehension of the mechanism of wound healing process. Research Support: Thecell network‐FRQS.