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Polymorphisms in Toll‐Like Receptors 2 and 5 Are Associated with Legionella pneumophila infection
Author(s) -
Zhang Fan,
Wang Suping,
Gao Xiangdong,
Wu Weiwei
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.647.2
Subject(s) - tlr2 , peripheral blood mononuclear cell , single nucleotide polymorphism , tlr5 , genotype , tlr4 , biology , real time polymerase chain reaction , population , legionella pneumophila , toll like receptor , immunology , microbiology and biotechnology , medicine , immune system , gene , in vitro , bacteria , innate immune system , genetics , environmental health
Objective we investigated whether single nucleotide polymorphisms (SNPs) in Toll‐like receptors2 (TLR2) and TLR5 affect the susceptibility of LD in a Han Chinese population by in vitro assay. Method Fifty four healthy subjects were genotyped for SNPs (TLR2 C597T [rs3804099] and TLR5 C1174T [rs5744168]). Peripheral blood mononuclear cells (PBMCs) were obtained from these subjects and were stimulated with live Legionella pneumophila (Lp) for 24 hours. The mRNA levels were determined using real‐time quantitative Polymerase Chain Reaction (RT‐qPCR) and the levels of TNF‐α, IL‐1β and IL‐6 were measured by enzyme‐linked immunosorbent assay (ELISA). Results After twenty four hours of Lp stimulation, the mRNA expression level of MyD88 was significantly lower in PBMCs with TLR2(C597T) CT/TT (P=0.0482) or with TLR5(C1174T)CC homozygotes (P=0.0223) comparing to PBMCs with other genotypes. Supernatant from culture of PBMCs with genotype TLR2(C597T) CT/TT or TLR5(C1174T)CC has higher levels of TNF‐α, IL‐1β and IL‐6 comparing to PBMCs with other genotypes after Lp‐stimulation. Conclusion Our findings suggest that both the TLR2(597CT/TT, rs3804099) and the TLR5 (1174CC, rs5744168) polymorphic variants are associated with resistance to LD in the evaluated Han Chinese population. This study was supported by the National Natural Science Funds of China (grant no. 30872154)