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Immune‐enhancing Effects of Polysaccharides Isolated from Cheonggukjang in Immunosuppressed Mice
Author(s) -
HONG HEEDO,
HAN CHUNJI,
CHO CHANGWON,
RHEE YOUNG KYOUNG,
NAM SOHYUN,
SUNG SUKYUNG,
KIM YOUNGCHAN,
KIM KYUNGTACK
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.643.8
Subject(s) - immune system , polysaccharide , galactose , chemistry , ethanol precipitation , immunology , microbiology and biotechnology , food science , biology , biochemistry
In this study, the crude polysaccharide (CHW‐0) was isolated from Cheonggukjang (Korean traditional fermented soybean paste) by hot water extraction, ethanol precipitation and dialysis. The major constituents in CHW‐0 were neutral sugar (69.5%), acidic polysaccharide (24.0%) and protein (6.5%). The neutral sugar was composed mainly of galactose (39.9 mole%), arabinose (31.9 mole%), and glucose (8.8 mole%). We also investigated the immune‐enhancing effects of CHW‐0 in 30 male Chinese Kun Ming mice immunosuppressed by treatment with cyclophophamide. The mice were divided into three groups, and treated by oral administration with CHW‐0 into two dosages (100 mg/kg and 200 mg/kg body weight) for 4 weeks. The results showed that CHW‐0 stimulated proliferation of splenic lymphocytes (T and B cells) in cyclophophamide‐induced immunosuppressed mice. CHW‐0 also markedly increased the natural killer‐cell activity and peripheral white blood cell number. This result demonstrated that CHW‐0 could restore deviated immune parameters in immunosupressed mice and can be utilized as an effective immune‐enhancer.